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The total protein amount is important information for quality control and protein values for the food and feed industry along with research facilities. Alpha Resources offers a variety of certified reference materials suitable for use with the Dumas Method.

The most efficient way to find the Nitrogen/Food Protein values is the Dumas Method which combusts samples using a high-temperature furnace in an oxygen-rich environment. The Dumas method is much easier to use, faster and fully automated compared to the Kjeldahl method. Also, as with Kjeldahl, it does not give a measure of true protein which requires different correction factors to obtain a correct value.

Dumas nitrogen determination requires well-homogenized samples, heated in a high-temperature furnace where the combustion takes place rapidly at over 1000 °C in the presence of pure oxygen. This produces mostly water, carbon dioxide and nitrogen as well as several oxides (NyOx). This gas mixture (most water is removed) is passed through a reduction chamber containing copper heated to around 650 °C. This converts nitrogen oxides into elemental nitrogen and collects the oxygen in excess. Different traps remove the residual water and carbon dioxide. Total nitrogen content is measured by a thermal conductivity detector.


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Latest News

Stevensville, Michigan, October 12, 2021 – Alpha Resources LLC, the largest manufacturer of aftermarket consumables and reference materials, is pleased to announce the following certified reference materials (CRM) have been released in Q3 of 2021. Please click to read more.

Stevensville, Michigan, July 28, 2021 – Alpha Resources LLC has been chosen as the successor for the customers of Crossings, Inc. Crossings, Inc. is an independent manufacturer, processor, and distributor of consumables for CHN/protein analyzers based in Lincoln, Nebraska. Click here to read the full story.